Western Blot:Article Title: Fetuin-A is a HIF target that safeguards tissue integrity during hypoxic stress
Article Snippet: Primary antibodies Target/protein Host species Clonality Application Source Alpha smooth muscle actin rabbit mono WB Abcam, ab32575 Aquaporin-1 rabbit poly IF SCBT, sc-20810 Aquaporin-2 rabbit poly IF J. Loffing18 Beta actin rabbit poly WB Abcam, ab8227 Caspase-3 (cleaved) rabbit poly IF Cell Signaling, 9661S CD11b-PE rat mono FACS eBioscience, 12-0112-82 CD11c-BV 605 hamster mono FACS BioLegend, 117333 CD68-APC-Cy7 rat mono FACS BioLegend, 137024 CD80-Pacific blue hamster mono FACS BioLegend, 104723 CD86-APC rat mono FACS BioLegend, 105011 CD206-FITC rat mono FACS BioLegend, 141703 Collagen 1 mouse mono WB SCBT, sc-293182 F4/80-PE-Cy7 rat mono FACS BioLegend, 123114 Fetuin-A goat poly IF, WB SCBT, sc-9668 Fetuin-A rabbit poly WB W. Jahnen-Dechent19 Fetuin-A rabbit mono IF, IHC Sino Biological, 50093-R022 14 Fibronectin mouse mono WB SCBT, sc-73611 Ncc rabbit poly IF J. Loffing20 Nephrin goat poly IF R&D, AF3159 Nkcc2 rabbit poly IF J. Loffing18 Phospho-Smad3 rabbit mono WB Cell Signaling, 9520S Smad3 mouse mono WB SCBT, sc-101154 Vimentin rabbit mono WB Cell Signaling, 5741T Secondary antibodies Target Host species Conjugate Application Source goat donkey Alexa Fluor® 647 IF Jackson, 705-605-147 goat donkey Cyanine CyTM2 IF Jackson, 705-225-147 goat donkey HRP WB Jackson, 705-035-147 goat donkey HRP IHC SCBT, sc-2304 mouse goat HRP polymer IHC Agilent, K4001 mouse donkey HRP WB Jackson, 715-035-151 rabbit goat HRP polymer IHC Agilent, K4003 rabbit donkey Cyanine CyTM3 IF Jackson, 711-165-152 rabbit donkey HRP WB Jackson, 711-035-152 rat donkey Alexa Fluor® 647 IF Jackson, 712-606-153 Supplementary Table 8 P-values for postnatal growth curve (Figure 1g).
FACS:Article Title: Fetuin-A is a HIF target that safeguards tissue integrity during hypoxic stress
Article Snippet: Primary antibodies Target/protein Host species Clonality Application Source Alpha smooth muscle actin rabbit mono WB Abcam, ab32575 Aquaporin-1 rabbit poly IF SCBT, sc-20810 Aquaporin-2 rabbit poly IF J. Loffing18 Beta actin rabbit poly WB Abcam, ab8227 Caspase-3 (cleaved) rabbit poly IF Cell Signaling, 9661S CD11b-PE rat mono FACS eBioscience, 12-0112-82 CD11c-BV 605 hamster mono FACS BioLegend, 117333 CD68-APC-Cy7 rat mono FACS BioLegend, 137024 CD80-Pacific blue hamster mono FACS BioLegend, 104723 CD86-APC rat mono FACS BioLegend, 105011 CD206-FITC rat mono FACS BioLegend, 141703 Collagen 1 mouse mono WB SCBT, sc-293182 F4/80-PE-Cy7 rat mono FACS BioLegend, 123114 Fetuin-A goat poly IF, WB SCBT, sc-9668 Fetuin-A rabbit poly WB W. Jahnen-Dechent19 Fetuin-A rabbit mono IF, IHC Sino Biological, 50093-R022 14 Fibronectin mouse mono WB SCBT, sc-73611 Ncc rabbit poly IF J. Loffing20 Nephrin goat poly IF R&D, AF3159 Nkcc2 rabbit poly IF J. Loffing18 Phospho-Smad3 rabbit mono WB Cell Signaling, 9520S Smad3 mouse mono WB SCBT, sc-101154 Vimentin rabbit mono WB Cell Signaling, 5741T Secondary antibodies Target Host species Conjugate Application Source goat donkey Alexa Fluor® 647 IF Jackson, 705-605-147 goat donkey Cyanine CyTM2 IF Jackson, 705-225-147 goat donkey HRP WB Jackson, 705-035-147 goat donkey HRP IHC SCBT, sc-2304 mouse goat HRP polymer IHC Agilent, K4001 mouse donkey HRP WB Jackson, 715-035-151 rabbit goat HRP polymer IHC Agilent, K4003 rabbit donkey Cyanine CyTM3 IF Jackson, 711-165-152 rabbit donkey HRP WB Jackson, 711-035-152 rat donkey Alexa Fluor® 647 IF Jackson, 712-606-153 Supplementary Table 8 P-values for postnatal growth curve (Figure 1g).
Immunohistochemistry:Article Title: Fetuin-A is a HIF target that safeguards tissue integrity during hypoxic stress
Article Snippet: Primary antibodies Target/protein Host species Clonality Application Source Alpha smooth muscle actin rabbit mono WB Abcam, ab32575 Aquaporin-1 rabbit poly IF SCBT, sc-20810 Aquaporin-2 rabbit poly IF J. Loffing18 Beta actin rabbit poly WB Abcam, ab8227 Caspase-3 (cleaved) rabbit poly IF Cell Signaling, 9661S CD11b-PE rat mono FACS eBioscience, 12-0112-82 CD11c-BV 605 hamster mono FACS BioLegend, 117333 CD68-APC-Cy7 rat mono FACS BioLegend, 137024 CD80-Pacific blue hamster mono FACS BioLegend, 104723 CD86-APC rat mono FACS BioLegend, 105011 CD206-FITC rat mono FACS BioLegend, 141703 Collagen 1 mouse mono WB SCBT, sc-293182 F4/80-PE-Cy7 rat mono FACS BioLegend, 123114 Fetuin-A goat poly IF, WB SCBT, sc-9668 Fetuin-A rabbit poly WB W. Jahnen-Dechent19 Fetuin-A rabbit mono IF, IHC Sino Biological, 50093-R022 14 Fibronectin mouse mono WB SCBT, sc-73611 Ncc rabbit poly IF J. Loffing20 Nephrin goat poly IF R&D, AF3159 Nkcc2 rabbit poly IF J. Loffing18 Phospho-Smad3 rabbit mono WB Cell Signaling, 9520S Smad3 mouse mono WB SCBT, sc-101154 Vimentin rabbit mono WB Cell Signaling, 5741T Secondary antibodies Target Host species Conjugate Application Source goat donkey Alexa Fluor® 647 IF Jackson, 705-605-147 goat donkey Cyanine CyTM2 IF Jackson, 705-225-147 goat donkey HRP WB Jackson, 705-035-147 goat donkey HRP IHC SCBT, sc-2304 mouse goat HRP polymer IHC Agilent, K4001 mouse donkey HRP WB Jackson, 715-035-151 rabbit goat HRP polymer IHC Agilent, K4003 rabbit donkey Cyanine CyTM3 IF Jackson, 711-165-152 rabbit donkey HRP WB Jackson, 711-035-152 rat donkey Alexa Fluor® 647 IF Jackson, 712-606-153 Supplementary Table 8 P-values for postnatal growth curve (Figure 1g).
Polymer:Article Title: Fetuin-A is a HIF target that safeguards tissue integrity during hypoxic stress
Article Snippet: Primary antibodies Target/protein Host species Clonality Application Source Alpha smooth muscle actin rabbit mono WB Abcam, ab32575 Aquaporin-1 rabbit poly IF SCBT, sc-20810 Aquaporin-2 rabbit poly IF J. Loffing18 Beta actin rabbit poly WB Abcam, ab8227 Caspase-3 (cleaved) rabbit poly IF Cell Signaling, 9661S CD11b-PE rat mono FACS eBioscience, 12-0112-82 CD11c-BV 605 hamster mono FACS BioLegend, 117333 CD68-APC-Cy7 rat mono FACS BioLegend, 137024 CD80-Pacific blue hamster mono FACS BioLegend, 104723 CD86-APC rat mono FACS BioLegend, 105011 CD206-FITC rat mono FACS BioLegend, 141703 Collagen 1 mouse mono WB SCBT, sc-293182 F4/80-PE-Cy7 rat mono FACS BioLegend, 123114 Fetuin-A goat poly IF, WB SCBT, sc-9668 Fetuin-A rabbit poly WB W. Jahnen-Dechent19 Fetuin-A rabbit mono IF, IHC Sino Biological, 50093-R022 14 Fibronectin mouse mono WB SCBT, sc-73611 Ncc rabbit poly IF J. Loffing20 Nephrin goat poly IF R&D, AF3159 Nkcc2 rabbit poly IF J. Loffing18 Phospho-Smad3 rabbit mono WB Cell Signaling, 9520S Smad3 mouse mono WB SCBT, sc-101154 Vimentin rabbit mono WB Cell Signaling, 5741T Secondary antibodies Target Host species Conjugate Application Source goat donkey Alexa Fluor® 647 IF Jackson, 705-605-147 goat donkey Cyanine CyTM2 IF Jackson, 705-225-147 goat donkey HRP WB Jackson, 705-035-147 goat donkey HRP IHC SCBT, sc-2304 mouse goat HRP polymer IHC Agilent, K4001 mouse donkey HRP WB Jackson, 715-035-151 rabbit goat HRP polymer IHC Agilent, K4003 rabbit donkey Cyanine CyTM3 IF Jackson, 711-165-152 rabbit donkey HRP WB Jackson, 711-035-152 rat donkey Alexa Fluor® 647 IF Jackson, 712-606-153 Supplementary Table 8 P-values for postnatal growth curve (Figure 1g).
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